In order to investigate the varied expression of peroxisome proliferator-activated receptor-γ(PPAR-γ) in hypertrophied ventricle induced by pressure overload, the abundance of PPAR-γ in ventricles of spontaneously hypertensive rats (SHR) were detected. Using age matched Wistar-Kyoto rats (WKY) as control, the body mass (BM), left ventricle (LV) wet mass (WM), interventricular septum (IS) WM and right ventricle (RV) WM from 4-week-old and 16-week-old SHR were detected respectively. Then the protein expression in ventricles of WKY and SHR was detected by immunohistochemistral techniques and Western blot, and the mRNA level was analyzed by reverse transcription-polymerase chain reaction (RT-PCR). The ratio of LVWM, ISWM and RVWM to BM in 4-week-old SHR were similar to that in 4-week-old WKY (P＞0.05), and the protein and mRNA level of PPAR-γ in ventricles of 4-week-old SHR were also similar to that of age matched WKY (P＞0.05). In 16-week-old SHR, the ratio of LVWM and ISWM to BM were higher compared with age matched WKY (P＜0.01), whereas there was no significant difference in RV (P＞0.05). The protein and mRNA level in LV and IS of 16-week-old SHR were great lower than that of 16-week-old WKY (P＜0.01), while in RV there was no significant difference of protein (P＞0.05), and a little decrease of mRNA (P＜0.05). The findings suggested that the expression level of PPAR-γ in hypertrophied ventricles induced by pressure overload (LV and IS in 16-week-old SHR) was significantly decreased, and the decreased expression of PPAR-γ in ventricles might contribute to ventricular remodeling in hypertension.