NGX6 is a candidate tumor suppressor gene which was isolated by location candidate cloning strategy. In order to explore the effect of NGX6, the mammal expression vector of NGX6/pcDNA3.1(+) was transfected into 5-8F cell （ with high ability of metastasis ） by liposome. The integration of the exogenous vector DNA and the expression of NGX6 were detected by Northern blot and RT-PCR respectively. The cytobiological characterization of positive clone was analyzed by growth curves of cells and soft agar assay. cDNA array techniques were used to profile the potential targets of NGX6. The results showed that 5-8F cells with overexpression of NGX6 grew slower than that of control, and NGX6 could influence the expression of cell cycle, cell adhesion and angiopoiesis molecules, such as up-regulating p19, catenin α2, desmoglein 1,down-regulating EphB4, TIE2, vitronectin. These data revealed that NGX6 might play a role in tumor metastasis in nasopharyngeal carcinoma (NPC).