Photodynamic therapy (PDT) has been widely used as an anticancer treatment in the clinic. However, the damage to the vessel induced by PDT, one of the most important aspects to tumour therapy, is not clearly understood. In order to study the effect of vascular damage in vivo during PDT, the chicken chorioallantoic membrane (CAM) tumor model by using stable high GFP-expressing human adenoid cystic carcinoma cell strain (ACC-M-GFP) is built for the experiment. The fluorescence imaging was used to show the location and the size of the tumor and treatment region. The dynamic parameter change of blood with different photodynamic doses in PDT was monitored in real time by laser speckle contrast imaging (LSCI) technique. The experimental results show that: the photodynamic effects are observably different at different photodynamic doses. It proved that: firstly the GFP-expressing tumor CAM model is extremely suitable for studying the photodynamic effect on the vessels, because of its convenience to locate the tumor and treatment region on the CAM. Secondly, with the aid of the LSCI technique, it is able to perform real-time monitoring of vessel structure and flow velocity change to identify the perfusion variation in PDT and to additionally investigate the damage to the vessels around the tumor induced by PDT.