Endolysins or lysins are cell-wall-hydrolysing enzymes synthesized during late gene expression in the lytic cycle of bacteriophage mutiplication and enable the release of progeny virions from infected cells through degradation of the bacterial peptidoglycan. The gene10 was amplified from purified DNA of Mycobacteriophage D29 by using PCR and was expressed with 6×His tag in E.coli. The soluble portion of recombinant protein was purified by Ni-NTA column and the activity was detected. The results showed that gp10 is a bi-functional enzyme, has chitinase and lysozyme activities. The cell wall degradation was observed by SEM.