When either massive damage is inflicted on the liver or liver regeneration after damage is compromised by a variety of toxins and carcinogens, the hepatocyte progenitor/stem cells which called “oval cells” is activated and differentiate into a variety of cell lineages including hepatocytes and biliary epitheliums. Those models used in the study of rodents' oval cells are obviously not suitable for the study on human hepatic oval cells. A new hepatic oval cells produced model suitable for human need be developed. Mouse embryonic stem cells (ES cells) were cultured and induced to develop into embryonic bodies. The induced group and the control group were set up then. Hepatocyte growth factor (HGF) and epidermal growth factor (EGF) was added to the culture medium of the induced group. The markers such as A6 antigen, which is expressed by hepatic oval cell, are detected by means of immunocytochemistry. As the descendants of mouse ES cells, hepatic oval cells sorted by FACS were detected by RT-PCR and transmission electron microscopy. The hepatic oval cells sorted by FACS were further cultured and tested the ability of bipotential differentiation by ICC and RT-PCR. It was firstly confirmed that the midterm phase of hepatic oval cells which are bipotential does occur during the course of the directional induction and differentiation of mouse ES cells into hepatic parenchyma cells. The differentiation ratios of hepatic oval cells from the induced group are significantly higher than that from the control group, and the maximal ratio from the induced group could be about 6.11%. HGF and EGF could promote the proliferation of hepatic oval cells derived from ES cells. About 4.59% of the cells sorted by FACS are Sca-1+/CD34+, and about 90.81% of the Sca-1+/CD34+ cells are A6 positive. Highly purified A6+/ Sca-1+/CD34+ hepatic oval cells derived from ES cells could be obtained by FACS. A new hepatic oval cells produced model suitable for human was developed.