The massive β-tricalcium phosphate (β-TCP) scaffold with a central tunnel was impregnated with the sheep mesenchymal stem cells and were continuously perfused with the complete α-MEM medium by a peristaltic pump for 7, 14 and 28 days. Histological study showed that the cells proliferated through the whole scaffolds under dynamic culture. The cells formed a continuous monolayer or multilayer in the first 7 days and some pores in the scaffold packed with cells in 14 days. Most of pores were almost fully filled with cells after 28 days' culture. And the cell coverage were various in various spaces of the scaffold. Unoccupied spaces were found in many macropores and cell death was found in some spaces after 14 days' culture. The CFD was used to simulate the flow conditions within perfused cell-seeded cylindrical scaffolds to give insight into the mechanisms of the cell growth phenomena. Relating the simulation results to perfusion experiments, the average fluid velocity (approximately 0.24～0.53 mm/s) and shear stress (approximately 0.0050～0.023 Pa) were found to correspond to increased cell proliferation within the cell-scaffold constructs.