To explore a new way to purify EPCs, hybridoma dish was used to isolate EPCs from rat bone marrow -derived cells,according to the morphology of endothelial progenitor cells colony-forming units (EPCs-CFUs) and special markers of EPCs. The bone marrow derived cells, from rat femoral bone and shinbone, were plated and cultured on hybridoma dish which was maded of polystyrene. Between 4th and 7th days in culture, stem cells colony-forming units were picked out respectively under microscope. Then one part of this cells was identified by immunofluorescence staining of CD133⁺vEGFR-2⁺, the special markers of EPCs. If the both markers of this part was positive, the rest part of the cells was continuously cultured for passaging. This method was named as “Micropore-Method”. The conspicuous stem cells colony-forming units were observed under microscope after four days in cluture, about 7% CFUs were CD133⁺/VEGFR-2⁺ positive EPCs-CFUs. After further culturing 7 days, Purity of cells with special markers of CD133⁺/VEGFR-2⁺/CD34⁺ was over 70% identified by flow cytometry. Passaging cells can form capillary tube like formation and differentiate to endothelial like cells expressing EC special marker vWF. It can be concluded that “Micropore-Method” is a new successful way to isolate EPCs from rat bone marrow.