This work was supported by grants from The National Science Foundation of China (30400241, 30672076) and Postdoctoral Science Funds of China (2005038478).
In order to obtain MicroRNA (miRNAs) expression profiles of human hematopoietic stem cells (HSCs), and to preliminarily investigate functions of HSC-correlative miRNAs, by using miniMACS magnetic beads and fluorescence-activated cell sorting (FACS), isolated hematopoietic stem cells (HSCs) were isolated from human umbilical cord blood and performed total RNA extraction. Next, using a microarray, miRNA gene expression profiles of HSCs were obtained. CFC assays were performed to research on differentiation-promoting function of miR-520h, enriched in HSCs. Results showed that CD34+ hematopoietic cells and HSCs were successfully isolated from human umbilical cord blood, and 31 HSCs-correlative miRNAs were screened by microarrray. Among them, 22 were low in HSCs, and 9 were high. The result of real time RT-PCR confirmed high expression of miR-520h in HSCs. CFC assays showed that miR-520h promotes differentiation of HSCs into progenitor cells. In conclusion, human HSCs have a set of specific miRNAs that contribute to regulation of HSCs functions, which pave the way for exploring the roles of miRNAs in development of hematopoiectic system.
SUN Jian-Guo, LIAO Rong-Xia, ZHANG liang, LOU Gui-Yu, ZHOU Du-Jin, CHEN Zheng-Tang. Screening of Hematopoietic Stem Cell-correlative MicroRNAs and Analysis of Their Differentiation-promoting Function[J]. Progress in Biochemistry and Biophysics,2007,34(11):1190-1196
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