Zebrafish, as a new model of animal, has been gradually accepted and widely used in biology research due to its special characteristics. Besides its convenience used in development research, the application in ethology is more and more extensively. Because of the transparency before 2 days post-fertilization, the eye size nearly half of the brain and its conspicuous circadian rhythm, zebrafish has been significantly applied in vision research. The organs of olfactory and auditory are both visible on the surface, such structure makes it very easy to detect the olfactory and auditory function by means of behavior experiments. Observation of motion is very convenience as zebrafish's propensity is active. It is also used more and more in social biology research. The behavior test of zebrafish is a simple and effective method to analyze the integrative neuronal function in vivo while some experimental models have been established which reviewed as follows.