Individuals with CCR5Delta32 mutant genotype can naturally resist human immunodeficiency virus type 1(HIV-1) infection. The mechanism is mainly due to CCR5Delta32 mutant protein expressed in the individual peripheral blood mononuclear cells (PBMCs), through trans-dominant negative (TDN) effect, which can inhibit two types of HIV-1 coreceptor (CCR5 and CXCR4) on the cell surface from producing. The recombinant lentiviruses, namely Lenti-CCR5Delta32, were generated and used to infect human PBMCs. HIV-1 infection of the PBMCs transduced with Lenti-CCR5Delta32 showed that CCR5Delta32 protein expressed in human PBMCs was able to inhibit R5, X4, and R5X4 HIV-1 infection. The result is expected to be used for the gene therapy on AIDS, which deserves further study.
AN Qun-Xing, LEI Ying-Feng, YANG Jing, ZHANG Xian-Qing, YI Jing, CHEN Rui, MU Shi-Jie. Inhibition of HIV-1 Infection by CCR5Delta32 Protein Expressed in Human PBMCs[J]. Progress in Biochemistry and Biophysics,2009,36(9):1141-1145
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