Monoclonal antibodies(mAbs) have the advantages of high specificity and stable expression, while avian IgY antibodies provide a series of advantages such as less interference reaction with non-specific proteins in immunoassays, and the immune system of birds reacts stronger with IgY production against highly conserved mammalian antigens than the mammalian immune system. Monoclonal IgY(mIgY) is a new antibody development concept to combine the both advantages of IgY and mAbs. It was reviewed the current research situation and improvement of mIgY, mainly focused on the theoretic background, techniques involved, research cases and application prospects of mIgY.