In order to explore the mechanisms of candidate liver tumor suppressor protein PIG11 induced apoptosis, eleven PIG11 binding proteins were first time identified in human hepatoma HepG2 cells, which heat shock protein 60 (Hsp60) is one of them. It has been confirmed by co-immunoprecipitation combined with Western blot analysis. Furthermore, using Western blot analysis, the protein expression of Hsp60 is down-regulated in pLXSN-PIG11-HepG2 cells group than that of pLXSN-HepG2 and HepG2 groups(n=3, P < 0.01), and over-expression of PIG11 induces translocation of Bax protein from cytoplasm to mitochondria in HepG2 cells. These results suggested that PIG11 protein can combine with Hsp60 in HepG2 cells, and the over-expression of PIG11 induce HepG2 cells apoptosis through mitochondrial pathway and the translocation of Bcl-2 family proteins Bax from cytoplasm to mitochondria maybe play an important role in the process.