Kindlins are a group of newly identified focal adhesion proteins, including Kindlin-1, Kindlin-2, and Kindlin-3. Kindlins possess high homogeneity and conservation evolutionarily, involving in the regulation of many important cellular physiological functions, such as cell migration, liferation, and differentiation. Through the interaction with the cytoplasmic domain of β integrin, Kindlins play important roles in cell-extracellular matrix adhesion, cell-cell junctions, cytoskeleton remodeling, and integrin-mediated bidirectional signaling. Abnormities of Kindlins are associated with the etiology and progression of some genetic diseases, cardiovascular diseases, and cancers. The further elucidation of the mechanisms regulating the cellular functions mediated by the interaction between Kindlins and integrins will not only improve our knowledge about cell adhesion and migration, but also the therapeutics of Kindlins-related diseases.