The cDNA of UCP5 gene was obtained by 3′ and 5′ RACE, the length was 1 611 bp. One A→T mutation was found at -1 567 bp of UCP5 gene. The mutation resulted in the change of transcript factors CdxA, HNF-1, Sox-5, GATA-2. Different genotypes were detected in 524 Jinhua× Pietrain pigs using PCR-RFLP, and the relationships between the genotypes and live weight, muscle weight of hind leg, fat weight of hind leg, average back-fat thickness, back-fat thickness in the 6th～7th rib, area of loin and leaf fat weight were analyzed. The statistical analysis showed that animals of the AA genotype had the highest live weight, which was significantly higher than that of BB genotype and obviously higher than that of AB genotype. For muscle weight of hind leg and hind leg fat weight, AA genotype was also higher than AB and BB genotype. By Real-time quantitative PCR, UCP5 expression was analyzed in heart, liver, spleen, lung, kidney, loin, abdominal adipose and cerebral tissues. The results showed that UCP5 existed in all tissues, and the expression of UCP5 was the majority in cerebral tissue and lung. The result of t-test showed that the distribution of UCP5 in loin of Dalan pigs was significantly higher than that of Jinhua.