This work was supported by grants from The National Natural Science Foundation of China(30670449, 30540079, 30970585), National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program", China(2009zx09301-001) and The State Key Laboratory of Molecular Biology
A basic research subject is the study on interactions between proteins, DNA and RNA. Majority of DNA and RNA-binding proteins are self polymerization-prone and may form aggregates that are difficult to bind DNA or RNA, thus hampering experiments. Labeling the proteins with fluorescein isothiocyanate significantly inhibits this tendency and markedly increases the binding efficiency of these proteins to bind nucleic acid molecules. This simple method has been utilized in the investigation on binding of C/EBPβ 3′UTR RNA by cytokeratin 18.
WANG Ying, SUN Da-Quan, LIU Ding-Gan. A Simple Method to Raise Efficiency of Interaction With Nucleic Acids of Self Polymerization-prone Proteins[J]. Progress in Biochemistry and Biophysics,2011,38(2):173-176
Copy® 2024 All Rights Reserved ICP:京ICP备05023138号-1 京公网安备 11010502031771号