To investigate the function of Laminin G domain (LG domain) of STGC3 in human nasopharyngeal carcinoma cell line CNE2. Recombinant plasmids pcDNA3.1(+)-STGC3 and pcDNA3.1(+)-STGC3^Δ1～42AA were respectively transfected into CNE2 cells by liposome-mediated transfection. Therefore, the CNE2/pcDNA3.1(+)- STGC3 and CNE2/pcDNA3.1(+)-STGC3^Δ1～42AA cell lines of stable expression STGC3 were established. Here, it is reported that deletion of LG domain in this STGC3 reduces the tumor suppression activity of it, as demonstrated by drawing growth curve, experimenting plate clone formation as well as detecting cell cycle distribution. The results showed: the ability of STGC3^Δ1～42AA suppressing CNE2 cell proliferation was obviously decreased to compare with wild type STGC3 (n=4, P < 0.05). These results indicate that the LG domain is necessary for the tumor suppression activity of the STGC3.