To explore the possibility of foreign gene expression in transgenic silkworm mediated by non-transposon vector, a hIL-28A gene was inserted into the insect cells expression vector pIZT-V5-His to generate recombinant vector pIZT/V5-His-hIL-28A, the vector was transferred into silkworm eggs by sperm mediated gene transfer, screening for gfp gene and verified by PCR and Dot blot hybridization. Transgenic silkworms were obtained after a specific band with the molecular mass of 25 ku could be detected in transgenic silkworm by Western blotting using an goat anti-hIL-28A antibody, and the content of hIL-28A in the G3 generation transgenic silkworms estimated by ELISA was approximately 0.198, 0.32, 0.238 ng/g in freeze-dried whole bodies, posterior silk glands and fat bodies, respectively. These results suggested that a heterologous gene could be integrated into silkworm genome by non-transposon vector and expressed successfully.