Autophagy extensively participate in physiological and pathological processes, and has been focused by contemporary biomedicine scientists in recent years. Transmission electron microscopy, immunofluorescence and immunoblotting techniques were common used in detection of autophagy. Deeper research needs more accurate detection of autophagy. Dysfunction of autophagy involves formation and degeneration of autophosome, accordingly, accurate and comprehensive evaluation of autophagy includes autophosome detection, as well as the fluency of autophagic degeneration, i.e. autophagic flux assay. Additionally, artificial up- or down-regulation of autophagy by drugs or gene interferences in in vitro or in vivo models has also been considered as important part of autophagy analysis. Any method currently used alone may not been as evidence of autophagy. More careful attention should be paid on results of any assays of autophagy, especially DO NOT interpret "increase or decrease of autophosome" (also "up- or down-expression of autophagy-related proteins") as "enhancement or attenuation of autophagic function".