Department of Nutrition and Food Hygiene, School of Public Health, Peking University,Department of Nutrition and Food Hygiene, School of Public Health, Peking University,Department of Nutrition and Food Hygiene, School of Public Health, Peking University,Department of Nutrition and Food Hygiene, School of Public Health, Peking University
This work was supported by the Foundation (2006BAD27B01) from the Ministry of Science and Technology of China
We examined the effect of myricetin on cell dysfunction in cytokine-induced pancreatic β cells and assessed whether Wnt signal pathway was the target of myricetin. RIN-m5f β cells were exposed to a combination of tumor necrosis factor-α, interleukin-1β, and interferon-γ, with or without myricetin pretreatment for 48 h. The cell viability, basal and glucose-stimulated insulin secretion and Wnt-signaling proteins were evaluated with methyl thiazolyl tetrazolium assay, radio immunoassay and Western blotting, respectively. The 48 h multiple-cytokine treatment decreased cell viability and glucose-stimulated insulin secretion, while increasing basal insulin secretion. Western blot analysis showed that Wnt-signaling proteins were decreased in cytokine-treated RIN-m5f cells. However, myricetin pretreatment protected against cytokine-induced cell death. In addition, myricetin (20 μmol/L) obviously decreased basal insulin secretion and increased glucose-stimulated insulin secretion in cytokine-treated RIN-m5f cells. Western blot analysis showed that Wnt-signaling proteins were increased after myricetin pretreatment. Therefore, myricetin might attenuate cell dysfunction in cytokine-induced RIN-m5F cells via the Wnt signal pathway, and the Wnt signal pathway might be used as a new target for protecting pancreatic β cells against cytokine-induced cell dysfunction and death.
DING Ye, DAI Xiao-Qian, ZHANG Zhao-Feng, LI Yong. Upregulation of Wnt Signal Pathway by Myricetin Attenuates β-Cell Dysfunction Induced by Cytokines[J]. Progress in Biochemistry and Biophysics,2013,40(1):50-56
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