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This work was supported by grants from The National Natural Science Foundation of China (81272298, 30871282, 30871365, 81172189 and 81171930), The Hunan Province Natural Sciences Foundation of China (10JJ7003), The Fok Ying Tong Education Foundation (121036), The Fundamental Research Funds for The Central Universities (2011JQ020), The Mittal Innovative Entrepreneurial Project of Central South University (11MX27) and The Open-End Fund for the Valuable and Precision Instruments of Central South University
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a burgeoning technique which combines Chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to detect protein-DNA binding events, histone modifications, nucleosomes positioning and DNA methylation on a genome-wide scale. Motivated by the tremendous progress in next-generation sequencing (NGS) technology, ChIP-seq offers higher resolution, less noise, and broader coverage than conventional microarray based ChIP-chip.With the decreasing cost of sequencing, ChIP-seq has become an indispensable tool for studying gene regulation and epigenetic mechanisms. In this review, we describe its latest advances, with an emphasis on issues related to data analysis and its application.
LIANG Fang, XU Ke, GONG Zhao-Jian, LI Qiao, MA Jian, XIONG Wei, ZENG Zhao-Yang, LI Gui-Yuan. ChIP-seq: a New Technique for Genome-wide Profiling of Protein-DNA Interaction[J]. Progress in Biochemistry and Biophysics,2013,40(3):216-227
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