College of Elementary Medicine Hebei United University,Tangshan Gongren Hospital,Tangshan 063000, China,Tangshan Gongren Hospital,Tangshan 063000, China,Tangshan Renmin Hospital,Tangshan 063000, China,Tangshan Qianxi Traditional Medicine Hospital, Tangshan 063000 China,College of Elementary Medicine, Hebei United University,Tangshan 063000, China,College of Elementary Medicine, Hebei United University,Tangshan 063000, China
This work was supported by a grant from Tangshan science and technology bureau for basic medical research (12140209A-44)
CD1d-restricted natural killer T cell (NKT) is a subset of T cells and plays an important role in the regulation of diverse immune responses. MicroRNA-mediated RNA interference is emerging as a crucial regulatory mechanism in the control of NKT cell development and function. Yet, roles of specific microRNA in the development and function of NKT cells is not completely understood. In this study, miR-150 knockout (miR-150KO) mice were adopted and the quantities of thymic and peripheral NKT cells were detected by flow cytometry. Cytokine production was detected by intracellular staining and ELISA. We found that miR-150 deletion resulted in the decreased number of thymic NKT cells, while peripheral NKT cells did not change in mice. However, activated NKT cells in miR-150KO mice produced more IFN-γ than that of wild type control (WT) mice. In addition, using B16BL6 melanoma mouse model, we found that miR-150 deletion enhanced the inhibitory effect of α-Galcer on the lung metastasis of melanoma cells. Our data provide new clues for the specific role of miR-150 in the development and function of NKT cells.
ZHENG Quan-Hui, ZHANG Ai-Hong, ZHENG Ai-Hua, YAO Wen-Bo, GAO Jin-Ming, ZHANG Qing-Bo, LI Juan. MiR-150 Deletion Increases IFN-γ Production of NKT Cell and Inhibits Lung Metastasis of Mice Melanoma Cells[J]. Progress in Biochemistry and Biophysics,2013,40(5):454-460
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