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Research Paper: Influence of MWNTs/PMMA Thin Film on Cellular Behavior of Cardiac Stem Cells From Brown Adipose
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Department of Advanced Interdisciplinary Studies,Institute of Basic Medical Sciences and Tissue Engineering Research Center,Academy of Military Medical Sciences,Department of Advanced Interdisciplinary Studies,Institute of Basic Medical Sciences and Tissue Engineering Research Center,Academy of Military Medical Sciences,Institute of Pharmacology and Toxicology,Department of Advanced Interdisciplinary Studies,Institute of Basic Medical Sciences and Tissue Engineering Research Center,Academy of Military Medical Sciences,Department of Advanced Interdisciplinary Studies,Institute of Basic Medical Sciences and Tissue Engineering Research Center,Academy of Military Medical Sciences,Department of Advanced Interdisciplinary Studies,Institute of Basic Medical Sciences and Tissue Engineering Research Center,Academy of Military Medical Sciences,Department of Advanced Interdisciplinary Studies,Institute of Basic Medical Sciences and Tissue Engineering Research Center,Academy of Military Medical Sciences,Department of Advanced Interdisciplinary Studies,Institute of Basic Medical Sciences and Tissue Engineering Research Center,Academy of Military Medical Sciences,Department of Advanced Interdisciplinary Studies,Institute of Basic Medical Sciences and Tissue Engineering Research Center,Academy of Military Medical Sciences

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This work was supported by grants from The Key Program of National Natural Science Foundation of China (31030032), National Natural Science Funds for Distinguished Young Scholar (31025013), National Basic Research Program of China (2011CB606206), National High Technology Research and Development Program of China (2012AA020506) and The National Natural Science Foundation of China (31100697)

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    Abstract:

    Considering the structure and electrophysiological properties of native myocardium, significant progress has been made in cardiac tissue engineering based on nano-conductive materials. Carbon nanotubes possess the good mechanical and electrical properties and previous study have showed carbon nanotubes can promote cardiac cells adhesion, proliferation and maturation, and enhance cell-cell electrical coupling. However, cell behavior of carbon nanotubes on brown adipose-derived cardiac stem cells (CSCs) has not been investigated. Here, we first prepared multiple-walled carbon nanotubes (MWCNTs) / polymethylmethacrylate (PMMA) thin film according to the reported study. And then, we explored the influences of MWNTs/PMMA thin films on cell viability, proliferation and cardiac differentiation of brown adipose-derived CSCs were evaluated. We demonstrated MWNTs/PMMA thin films has no obvious effect on cell viability and proliferation of brown adipose CSCs compared to those on gelatin thin film. Furthermore, we observed brown adipose CSCs on MWNTs/PMMA thin films exhibited significantly higher amounts of α-actinin and connexin43. By transmission electron microscopy, we found that carbon nanotubes were in direct contact with cell membranes and regulated cell behavior. We first explored the influence of carbon nanotubes on brown adipose-derived CSCs and found that carbon nanotubes promoted the cardiac differentiation of brown adipose-derived CSCs. This study may be benefit to promote the therapeutic application of carbon nanotubes in myocardial infarction.

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DUAN Cui-Mi, SUN Hong-Yu, YANG Ye, LIU Zhi-Qiang, TANG Rong-Yu, WANG Chang-Yong, WANG Chun-Lan, FENG Lan-Lan, ZHOU Jin.Research Paper: Influence of MWNTs/PMMA Thin Film on Cellular Behavior of Cardiac Stem Cells From Brown Adipose[J]. Progress in Biochemistry and Biophysics,2013,40(10):1070-1076

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History
  • Received:June 21,2013
  • Revised:July 31,2013
  • Accepted:August 14,2013
  • Online: October 24,2013
  • Published: October 20,2013
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