Xi'an Jiaotong University,Xi'an Jiaotong University,Lawrence Berkeley National Laboratory,Xi'an Jiaotong University,Xi'an Jiaotong University,Lawrence Berkeley National Laboratory
This work was supported by grants from U.S. Department of Energy (DE-AC02-05CH11231), U.S. NIH (NHLBI, #1R01HL115153) and The National Natural Science Foundation of China (11374237, 11504287)
Antibody (also named as an immunoglobulin, Ig), a most important macromolecule for immune response in human body, has been developed as macromolecular drug to treat the cancer and immune diseases. Understanding of antibody three-dimensional (3D) structure and fluctuation could be an important step for further understanding and controlling the antibody pharmacological function. However, the study is limited by antibody flexible structure. In this paper, we reviewed the current research progresses on structural study of human IgG1 antibody conducted by our recently developed individual-particle electron tomography (IPET) method. The review includes the sample preparation method, basic logic of image processing strategy, 3D analysis and its application in antibody-drug conjunction and antibody structural fluctuation. We also discussed the strengths and weakness of the technique.
ZHANG Xing, LIAO Yu-Heng, TONG Hui-Min, ZHANG Lei, ZHANG Sheng-Li, REN Gang.3D Reconstruction and Structural Study of IgG1 Antibody by Individual-particle Electron Tomography[J]. Progress in Biochemistry and Biophysics,2016,43(9):839-849
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