Glycosylation is one of the most common and important post-translational modifications(PTMs) of proteins in living organisms. More than 50% proteins are glycosylated among mammals. Glycoproteins are widely distributed in cell membrane surface and body fluids, and play critical roles in biological processes. With the rapid development of biological mass spectrometry technique, as well as biological database and analysis software for glycan structure elucidation, glycoproteomics research field is booming soon. This article summarized the most commonly used bioinformatic resources in literatures, including new developed databases, MS technique and methods.