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Review: Progress in Understanding of Glycan Structure Analysis Based on Mass Spectrometry
Received:January 04, 2017  Revised:June 16, 2017
Key words:glycomics, glycan, mass spectrometry, multistage mass spectrometry, structure analysis
Fund:国家重点基础研究发展计划(973)(2013CB910100),国家自然科学基金(31270909),国家高技术研究发展计划(863) (2014AA021101)和二维微流控糖芯片分析仪的研制(YZ201249)资助项目
Author NameAffiliationE-mail
ZHAO Ke-Li Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
University of Chinese Academy of Sciences, Beijing 100049, China 
promet14@hotmail.com 
HUANG Chun-Cui Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China huangchuncui111@163.com 
WU Hong-Mei Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
GuangDong Bio-healtech Advanced Co., Ltd., Foshan 528000, China 
wuhongmei@moon.ibp.ac.cn 
LI Yan Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
University of Chinese Academy of Sciences, Beijing 100049, China 
yanli001@gmail.com 
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Abstract:
      Glycomics represents a novel omics technology developed after the genomics and proteomics, which focus on the glycan structure and function.As a group of biomolecule, glycans provide and store energy within the cell, and play many significant roles in physiological and pathological processes, such as cell-cell recognition and inflammatory and autoimmune diseases.Structural analysis of glycan is the bottleneck of glycomics research, due to their macro-heterogeneity (site occupancy) and micro-heterogeneity (different glycoforms attached to one glycosylation site). Comparing with traditional analysis approaches, mass spectrometry has become the method of choice for structural analysis methods in glycan, thanks to its ability to require minute amounts of sample, gain more different information and to be used in combination with high-throughput analysis methods. This paper summarizes the advance in our understanding of glycan structures using mass spectrometry (MS), including multistage mass spectrometry (MSn), LC-MS and CE-MS.
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