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Research: Comparative Study on Milk Oligosaccharides in Buffalo and Cow Colostrum Milk
Received:July 19, 2017  Revised:August 23, 2017
Key words:milk oligosaccharides, colostrum milk, cow, buffalo, UPLC-ESI-Q-TOF-MS
Fund:
Author NameAffiliationE-mail
MENG Xian-Pu Beijing Friendship Hospital Affiliated to The Capital University of Medical Sciences, Beijing 100050, China xianpumeng@126.com 
JIAO Si-Ming Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100095, China  
QIN Sheng-Qi Beijing Friendship Hospital Affiliated to The Capital University of Medical Sciences, Beijing 100050, China  
YANG Xian-Bei Beijing Friendship Hospital Affiliated to The Capital University of Medical Sciences, Beijing 100050, China  
LI Jian-She Beijing Friendship Hospital Affiliated to The Capital University of Medical Sciences, Beijing 100050, China  
WEI Jin-Hua Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100095, China  
WANG Zhuo Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100095, China  
DU Yi-Guang Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100095, China ygdu@ipe.ac.cn 
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Abstract:
      Milk oligosaccharides, which are free oligosaccharides in the mammalian milk, is one of the main composition in milk. Recent studies suggested that milk oligosaccharides improved immune function, and showed prebiotics as well as anti-infection actives. Milk oligosaccharides is also important for the intestinal development, neural development of infants. Buffalo (Bubalus bubalis) milk is the second important milk source besides cow milk. It was also well accepted that buffalo milk was rich in nutritional content and was a high quality dairy product with good taste. However, researches on buffalo milk oligosaccharides was limited and only worked on American buffalo. On the other hand, milk oligosaccharides on Chinese buffalo is lacking. In the present study, colostrum milk samples from Guangxi buffalo were purified with solid phase extraction and modified by Aniline (Bn) derivative reagent. The sample was further purified by UPLC-ESI-Q-TOF-MS. The oligosaccharide components in buffalo colostrum were determined and compared with the cow colostrum milk. 19 kinds of oligosaccharides were found in cow colostrum and 9 in buffalo colostrum. Moreover, in both two kinds of colostrum, neutral disaccharide m/z 385.15, neutral trisaccharide m/z 547.21 and acidic oligosaccharide m/z 635.23 were the main composition. In general, the proportion of neutral oligosaccharides in buffalo milk was higher than that in cow milk, with a ratio of 88.88% and 63.16%, respectively.
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