Reviews and Monographs: Advances in Optical Microscopic Imaging for Visualizing Liver Immunology In vivo
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1 Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology;2 MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology,1 Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology;2 MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology,1 Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology;2 MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology,1 Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology;2 MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology

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This work was supported by grants from The Major Research Plan of The National Natural Science Foundation of China (91542000, 91442201), The National Natural Science Foundation of China (81501593), National Science Fund for Distinguished Young Scholars (81625012), and the China Postdoctoral Science Foundation (2015M572148)

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    Abstract:

    Optical molecular imaging, which greatly promoted the development of immunology, is one of the best methods to investigate cell morphology, movement and function in a complex regional environment in vivo. The liver is a vital organ for maintaining body’s metabolism and detoxification, and it as an immunological organ. Understanding the basic feature and function of liver immunology is significant for prevention and treatment of liver disease and associated systemic diseases. Visualization immune response under physiological or pathological condition in vivo, providing spatial-temporal and dynamic information of multiple cells involved in key events and their interactions, can greatly enrich the knowledge of liver unique immune response. This review will focus on the current liver intravital imaging techniques and methods, and the application of optical microscopic imaging techniques in liver immunology, such as multiphoton excitation confocal microscopy and spin-disk confocal microscopy, and future development directions and challenges of in vivo liver imaging are prospected.

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LIN Qiao-Ya, HUANG Song-Lin, LUO Qing-Ming, ZHANG Zhi-Hong.Reviews and Monographs: Advances in Optical Microscopic Imaging for Visualizing Liver Immunology In vivo[J]. Progress in Biochemistry and Biophysics,2017,44(12):1056-1065

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History
  • Received:October 19,2017
  • Revised:October 19,2017
  • Accepted:November 06,2017
  • Online: December 19,2017
  • Published: December 20,2017