Cryopreservation will cause osmotic damage, toxic damage and ice crystal damage to oocytes, thus it is difficult to improve the quality of the frozen oocytes. In this paper, microfluidic and stepwise cryoprotectant (CPA) loading-unloading protocols were combined with three kinds of cryopreservation devices (OPS, QC and Cryotop) to cryopreserve the porcine oocytes for the first time. Moreover, PDMS-transparent ceramic and PDMS-glass integration microfluidic chips were employed for oocytes cryopreservation for the first time, the survival rate and development rate of cryopreserved oocytes were used to evaluate optimal cryopreservation protocol. Finally, early apoptosis rate, intracellular reactive oxygen species (ROS) and mitochondrial membrane potential of oocytes were analyzed. The results showed that the survival rate and cleavage rate of frozen oocytes by microfluidic loading-unloading of CPAs were significantly higher than traditional loading-unloading group. Microfluidic method could reduce the early apoptosis rate, intracellular ROS and mitochondrial damage, improve the quality of frozen oocytes. The survival rate and cleavage rate of oocytes cryopreserved by PDMS-transparent ceramic integrated chip were not significantly different from traditional OPS method. Microfluidic chip technology provides a new idea for oocytes cryopreservation and has good application prospects.