Savaid School of Medicine, University of Chinese Academy of Sciences, Beijing 100049, China
This work was supported by a grant from The National Natural Science Foundation of China (31271522).
The centrosome is the principal microtubule organizing center in most animal cells. It ensures orderly cell cycle progression with accurate chromosome segregation. We have previously reported that the centrosomal protein Centlein functions as a molecular linker between C-Nap1 and Cep68 to maintain centrosome cohesion. To explore novel function of Centlein, in this study, we generated Centlein knockout cell lines, and performed RNA-seq and data analysis on Centlein knock out and control cells, in parallel. Ablation of Centlein upregulated PLK1, CCNB1, CCNA2 and CDC20, and promoted cell cycle progression. PLK1 protein, found elevated in Centlein knock out cells, interacted with Centlein in vivo. We propose that centrosomal PLK1 exerting control over the cell cycle relies upon the interaction with Centlein.
YANG Ling, ZHANG Ying, YUAN Li. Loss of a Centrosomal Protein, Centlein, Promotes Cell Cycle Progression[J]. Progress in Biochemistry and Biophysics,2019,46(7):689-698
Copy® 2024 All Rights Reserved ICP:京ICP备05023138号-1 京公网安备 11010502031771号