ABC(ATP-binding cassette) transporters are members of the largest superfamily of membrane transporters which modulate the transmembrane of various substrates against a chemical gradient via the energy by ATP hydrolysis. All organisms harbor numerous ABC proteins in different cellular compartments such as the plasma membrane, vacuoles, mitochondria and peroxisomes. Typically, ABC transporters are consisted of transmembrane domains(TMDs) and nucleotide-binding domains(NBDs) to bind their substrates and ATP respectively. NBDs, as the power engine of the ABC transporters, mediate the ATP binding and hydrolysis, whileTMDs determine what ligands can be recognized. The ABC proteins, in most cases, were initially discovered during the investigation on resistance to a multitude of drugs, including PDR(pleiotropic drug resistance) and MDR (multidrug resistance). In the present article, we reviewed the research advances of the structure and transmembrane transport mechanism of ABC transporters and their roles in plant pathogenic fungi.