1) The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen 518052, China;2)Department of Radiation Physics, The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou 310022, China;3)Zhejiang Key Laboratory of Radiation Oncology, Hangzhou 310022, China
This short communication is to report the inhibitory effect of deuterium oxide (D2O) on germination and culturability of bacterial spore. Germination was triggered by L-alanine under ambient condition and in situ monitored by Tb3+-dipicolinic acid assay, phase contrast microscopy and absorbance measurement. Germination kinetics were characterized by final germination level, germination half time, and germination speed. The culturability of germinated spores was evaluated by enumerating colony-forming units. Results revealed that D2O retarded the whole germination process and reduced the culturability but didn’t affect the final germination level. We postulate that D2O enhanced the stability of some spore-specific signaling proteins.
CHANG Jinhui, WU Wen Jie. Effect of Deuterium Oxide on Bacillus atrophaeus Spore Germination[J]. Progress in Biochemistry and Biophysics,2021,48(10):1196-1203
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