Objective Repetitive transcranial magnetic stimulation (rTMS) has been widely used in clinics to realize the treatment of neurological and psychiatric diseases, especially in depression and pain. In recent years, many studies have applied it as an adjuvant approach to treatment of Parkinson’s disease (PD) in order to relieve the patient’s motor symptoms and improve motor function. At present, the unified Parkinson’s disease scale and motor task are used to evaluate the adjuvant treatment effect of rTMS on motor symptoms, but there are few studies exploring the regulatory mechanism of high-frequency rTMS stimulation.Methods In this study, 10 Hz rTMS over the primary motor cortex (M1) on the contralateral side of the limb symptom onset side in 16 PD patients. By comparing the changes of brain neuron activity and the interactions between brain function before and after stimulation, we investigated the regulatory effect of repeated sessions of M1 high-frequency rTMS on brain neuron activity in PD patients.Results The results showed that after 10 d of M1 stimulation, beta oscillations increased and gamma oscillation significantly decreased (P<0.05) in motor cortex, and the connectivity between the frontal and parietal cortex was reduced (P<0.05).Conclusion It shows that 10 Hz rTMS mainly changes the beta and gamma rhythm in PD patients. The changes of beta and gamma oscillations in motor cortex may be related to the improvement of motor function, and the changes in prefrontal gamma oscillations may be due to inhibits abnormal neuron firing activities and modulates the patient’s motor control function.