Department of Laboratory Medicine, The First Affiliated Hospital of Xi’an Medical University, Xi’an 710077, China
R446;Q7
This work was supported by grants from Health Committee of Shaanxi Province, Joint Project (Class B) (2022B009), Xi’an Science and Technology Bureau Project (Medical Research Project of Xi’an Innovation Capability Foundation project) (22YXYJ0126), and Project of Scientific and Technological Innovation Team of Xi’an Medical College (2021TD14).
CRISPR/Cas is not only an important gene editing tool, but also an effective molecular diagnostic tool. Presently, based on the CRISPR/Cas, a series of molecular diagnostic sensor systems have been established, which are widely used in nucleic acid and non-nucleic acid detection. There have been series reviews of nucleic acid molecular diagnostic sensor system based on CRISPR/Cas. However, there is no systematic review of CRISPR/Cas-based non-nucleic acid detection system. This paper is to review CRISPR/Cas-based non-nucleic acid molecular sensor diagnosis system of characteristics and principles. CRISPR/Cas system of non-nucleic acid molecular sensor diagnosis system is divided into two type, CRISPR/Cas12 and CRISPR/Cas13. Based on CRISPR/Cas12a, detection system of exosomes, CaT-SMelor small-molecule compounds, cell-free biosensors, fDNA-based small-molecule compounds, melamine and ochratoxin was established. Another large class of non-nucleic acid molecular diagnostic sensor systems that based on CRISPR/Cas13 is alkaline phosphatase (ALP) activity and pathogen detection system. The advantages of CRISPR/Cas of molecular diagnosis in vitro have been demonstrated. Therefore, the basic work flow, detection principle and characteristics of non-nucleic acid molecular diagnosis based on CRISPR/Cas molecular diagnosis sensor system are reviewed in this paper. It is expected to provide a basis for the application of CRISPR/Cas-based molecular diagnostic system in in vitro diagnosis.
LI Zhuo, ZHOU Jian, WANG Xin, XU Pu. Application of CRISPR/CAS Molecular Diagnostics Biosensor in Non-nucleic Acid Molecular Diagnosis[J]. Progress in Biochemistry and Biophysics,2023,50(8):1797-1805
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