DNaseⅠ footprinting is labor intensive, time consuming and the target protein should be purified to some extent.Solid-phase DNaseⅠ footprinting can enrich a sequence specific protein by target DNA immobilized onto paramagnetic beads. After DNaseⅠ digestion, separate the products by sequencing PAGE. This new method is quick ,simple and repeatable,which can minimize the exposure of the researcher to radiation. The solid-phase approach may also facilitate the analysis of factors using crude protein mixtures.