A new method for measuring the anion transport activity of Band 3 in the erythrocyte inside-out vesicles(IOVs)was presented.Impermeable IOVs containing 80mmol/L dipicolinic acid (DPA)were obtained by pressing the IOVs through a No.9 gauge needle in DPA- containing medium.Efflux of DPA from the IOVs into Tb3+-containing transport buffer was monitored fluorometrically. The technique is highly sensitive, reproducible and much convenient as well.
Feng Chang, Guan Zhiying, Tu Yaping. A New Method for Measuring Anion Transfer Across Membranes of Red Cell Inside-Out Vesicles[J]. Progress in Biochemistry and Biophysics,1995,22(2):154-158
Copy® 2024 All Rights Reserved ICP:京ICP备05023138号-1 京公网安备 11010502031771号