Isolation and Purification of Human Liver-Specific F Antigen and Preparation of Its Antiserum

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Isolation and Purification of Human Liver-Specific F Antigen and Preparation of Its Antiserum
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Abstract:

Human fresh livers were treated by homogenization and centrifugation,then were purified by sephadex-G200 column chromatography,DEAE cellulose column chromatography,and polyacrylamide gel electrophoresis,pure human liver F antigen was obtained.Specific antisera were prepard by immunizing guinea pigs with the purified human F antigen.

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Wang Junjun, Zao Zongnong, Zhuang Yiyi, Qiu Tinggang, Lu Wei. Isolation and Purification of Human Liver-Specific F Antigen and Preparation of Its Antiserum[J]. Progress in Biochemistry and Biophysics,1995,22(2):186-188

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Received:February 15,1994
Revised:May 21,1994
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