The sequence of a defined DNA primer can be detected easily by a method described as below. A PCR (polymerase chain reaction) was carried out with the primer to be detected and one of the M13 universal sequencing primers. The resulting PCR fragment was purified and inserted into a pUC-18 or pUC-19 at the multicloning sites. Then the sequence of the primer to be detected can be exactly known by sequencing the recombinated plasmid with a M13 universal sequencing primer.
Zhu Liuqin, Wu Yujie. A Method to Confirm the Sequence of a Defined DNA Primer[J]. Progress in Biochemistry and Biophysics,1996,23(3):284-285
Copy® 2024 All Rights Reserved ICP:京ICP备05023138号-1 京公网安备 11010502031771号