Total gangliosides were isolated from the liver of healthy rabbit, pig, canine, and canine erythrocytes. After high performance thin layer chromategraphy of the total gangliosides, the chromatograms were scanned by TLC scanner, the percentage contents were obtained. On the basis of lipid-bound sialic acid contents, the authors found that canine erythrocyte had the highest GM₃ content. GM₃ isolated from compacted canine erythrocytes with a yield of 351.0μg per ml and the purity was 92.2％.