In this paper, we have studied the changes of F-actin assembly in ascitic liver cancer cells of mouse under the action of extrinsic laminin and cis-platin. The results shows that after the extrinsic laminin combined with its receptors they can promote the assembly of F-actin, resulting in the increase of the amount of the F-actin, that cis-platin, one of the multiple-targeted antitumor drugs, inhibits the assembly of F-actin after linking with F-actin and that under the action of laminin and Cis-platin, the assembly tends to stability and the amount of the F-actin remains unchanged. This study gives a direct evidence for the opposite action of extrinsic laminin and cis-platin on the assembly of F-actin in ascitic liver cancer cells of mouse.