用氯化镉诱导鲫鱼(Carassius aurattus),应用层析介质 Sephacryl S 体系从鱼肝脏中提取金属硫蛋白(MT),并对其性质进行研究.实验表明鲫鱼 MT 的性质与哺乳动物 MT 的性质十分相似.分子量约10 000,含丰富的半胱氨酸,较多的赖氨酸,不含芳香族氨基酸.紫外250nm 处强吸收,280nm 处几乎没有吸收。鱼 MT 含7个金属每分子,并含有20个巯基.测得鲫鱼 MT 等电点 pI 在5.6左右.
The metallothionein(MT)was purified from the liver of Carassius auratus induced by exposure to cadmium chloride and characterized.It shows very similar to mammalian metallothioneins in all of its properties.The MT of the fish contains high cysteine contents(30%)and with an absence of aromatic acid residues.The thiol groups of the cysteine residues combine with the cadmium in SH/Cd molar ratio of 3∶1 and produce a characteristic absorption maxium at 250 nm,minimum at 280 nm.The metal analyses reveal only traces of zine and copper in addition to cadmium. The molecular weight is determined to be about 10 000 by gel filtration on HPLC and SDS-PAGE method. The isoelectric point of fish MT is demonstrated to be about 5.6.
任宏伟,王文清,茹炳根,李令媛,潘爱华,王英彦.鲫鱼金属硫蛋白的提纯及性质研究[J].生物化学与生物物理进展,1993,20(4):281-285
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