采用交变脉冲电场凝胶电泳和碱变性交变脉冲电场凝胶电泳方法,分析了棉病囊霉酵母菌及其2个不同的突变菌株的核型,得知此菌株含有5条染色体 DNA,而2株突变体的染色体 DNA 都没有大片段的缺失或双链断裂,但其稳定性不如野生型菌株的 DNA,而且存在单链断裂等碱不稳定性位点.
Pulsed-field gel electrophoresis and alkaline pulsed-field gel electrophoresis were used to analyze the chromosome DNAs of Ashbya gossypii and two kinds of its mutant strains CMG and JMG,which were obtained by γ-ray and Helium-Neon laser irradiation,respectively.The experiments resulted in the conclusions that AG contains five chromosome DNAs;and that no double-stranded breaks or loss of large DNA fragments but alkaline labile sites existed in both the mutant strains.
杨素红,葛忠良.用脉冲电场凝胶电泳分析棉病囊霉及其突变体的染色体DNA[J].生物化学与生物物理进展,1993,20(4):286-288
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