小鼠腹水型肝癌细胞对竹红菌乙素的摄取和滞留
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Uptake and Retention of Hypocrellin B by Murine Ascitic Hepatoma Cells
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    摘要:

    采用 SDS 胶束增溶法较详细地研究了小鼠腹水型肝癌细胞对竹红菌乙素的摄取和滞留过程.从实验结果表明:a.细胞摄取乙素与弗伦德里希吸附等温式符合较好,细胞的饱和吸附量为每个细胞吸附1.23×108乙素分子.b.摄取速度很快,37℃下3min 即可达到饱和.c.被细胞摄取的乙素较难重新释放到溶液,而且未发生明显代谢变化.d.血清可携带乙素在细胞间传递.e.乙素趋向于进入细胞膜双分子层的深处.还结合实验结果对所采用的 SDS 胶束增溶法进行了评述.

    Abstract:

    The uptake and the retention of hypocrellin B(HB)by murine ascitic hepatoma(AH)cells was measured as function of incubation time.The data of uptake fitted the Freundlich adsorption isotherm very well over a wide range of hypocrellin B concentrations.The uptake occured very quickly and reached the maximun in 3 min at 37℃.The adsorped HB was quite difficult to be released to phosphate buffered solution whereas the releasing process was enhanced significantly by the addition of serum into the bath medium.The fluorescence quenching experiments using fatty acid probes located at different depths of cellular membranes indicated that the HB molecules have the trend inserting into the deep hydrophobic domains.

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孙继山,张莉,乐加昌.小鼠腹水型肝癌细胞对竹红菌乙素的摄取和滞留[J].生物化学与生物物理进展,1993,20(4):289-293

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  • 收稿日期:1992-05-11
  • 最后修改日期:1993-02-03
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