The nucleotide sequence of bovine liver tRNA^Ile was determined by both the partial hydrolyzation in water and the Donis-keller method.The tRNA^Ile was consisted of 77 nucleotides and relatively rich in GC base pairs. The acceptor stem of the tRNA^Ile was characteristic of G5 . G69 mismatch. Forthermore, the cloverleaf structure of tRNA^Ile was showed according to the Holley mode as well as the amount of free energy in the each of stems versus loops.