由fd噬菌体与质粒重组载体噬菌粒(phagemid)构建大容量,高效筛选的表面表达抗体基因片段文库,经突变、模仿体内B细胞亲和力成熟过程(affinity maturation)以免疫亲和层析筛选高亲和力特异抗体片段.取代单克隆抗体,应用于免疫分析诊断.
Construction of complex antibody libraries that expressed soluble antibody fragments on the surface of fd-phagemid with high screening efficiency subjected to rounds of in vitro mutadons. The individual antibody gene can then be affinity matured by emulating the process that occurs in B-cells in vivo .The affinity matured antibody fragments are selected for their ability to bind antigen after phage recovery. This novel recombinant DNA methods may replace the technology of using mice and hybridoma for the selection and production of antibodies.
张志文,邹长江.单克隆抗体生产的新时代[J].生物化学与生物物理进展,1994,21(2):139-143
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