用月桂酸对超氧化物歧化酶进行化学修饰以改善其稳定性.活化的月桂酸与SOD在40℃碱性条件下反应1h,再用Sephacryl S-200柱进一步纯化.修饰SOD活性回收率93%,比活为6000U / mg.具有较强的稳定性,基本上消除了免疫原性并明显地延长了在血液中的半衰期;该产品适用于化妆品和食品.
Superoxide dismutase is modified with lauric acid to improve its stabillity. Activated lauric acid was reacted with bovine Cu, Zn-superoxide dismutase at 40℃ for 1h and it was further purified by a Sephacryl S-200 colum. There are 93% recovery of enzyme activity from the resulting lauric acid-superoxide dismutase conjugate. The specific activity of this enzyme product was 6000U / mg. The modified enzyme showed enhanced stability, substantially free from immunogenicity and prolonged its halflife in blood. As a consequence. it can beneficially be used as an enzyme for cosmetics and food industry.
阎家麒,谢文正.月桂酸修饰超氧化物歧化酶的制备及其性质研究[J].生物化学与生物物理进展,1994,21(2):154-157
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