首次报道了用乙醇作梯度洗脱有机相分离PTC-氨基酸的新方法.同乙腈相比,乙醇毒害性小、价廉、易得.在优化的色谱条件下,乙醇洗脱分辨率、灵敏度、准确度均佳,可成为PTC-氨基酸分析的一种极有前途的新方法.
Analysis of phenylthiocarbamyl (PTC) amino acids with ethanol as organic eluent is described. Compared with acetonitrile elution system, ethanol is less toxic, easier to obtain and much cheaper. Under optimized chromatographic conditions, the resolution, sensitivity and accuracy are excellent.
朱曙东,赵惠仁,赵昇皓.利用乙醇系统RP-HPLC分析PTC-氨基酸[J].生物化学与生物物理进展,1994,21(2):162-165
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