Simultaneous and rapid purification of total cytoplasmic RNA and genomic DNA from small numbers of transfected mammalian cells
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摘要:
采用4mol / L LiCl将DNA和RNA分相，建立了同时从少量转染细胞中快速提取细胞总RNA和大分子基因组DNA的方法.与以前的方法相比，本法快速、简便、经济，尤其适合应用在哺乳动物细胞基因表达与调控的研究中．
Abstract:
A protocol by using 4 mol / L LiCl phasing the DNA and RNA could lead to simultaneous and rapid purification of total cytoplasmic RNA and genomic DNA from small numbers of transfected mammaliam cells. Comparing with other methods, this protocol shows rapid. easy and economic, and can be used in many aspects especially in the studies of mammalian cell gene expression and regulation.
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