近年来,随着杆状病毒表达系统的普遍应用,阳性重组病毒筛选方法也有了很大的改进,从过去的经验性的ocu+/ocu-空斑表型筛选发展到根据颜色差异,药物抗性,抗生素抗性等等筛选重组病毒.同源重组过程也可在大肠杆菌或酵母甚至体外进行,大大提高了重组率,由于重组率是如此之高,有些方法可以免去繁琐的空斑选择,很快得到重组病毒的纯培养.文章对各种筛选方法进行了比较,并指出各自的优缺点.
Several selection methods for positive recombinant baculoviruses havebeen developed in the past years. The traditional plaque morphology difference method has been and will be replaced gradually by new methods based on drug resistance,antibiotic resistance etc,Some methods allow the homologous recombination process to be taken place in E.coli,in Saccharomyces cercoisiae or even in vitro. Owing to the greatly increasing frequency of positive recombinant virus,the stocks of positive recombinant virus are obtained easily. The advantages and disadvantages of each method are discussed.
朱反修,王福山,齐义鹏.昆虫杆状病毒表达系统中阳性重组病毒的筛选[J].生物化学与生物物理进展,1995,22(1):35-39
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