Degenerate oligonucleotide primers MY 11/9(Manos),GP 5/6(Snijders) were used for in vitro amplification by polymerase chain reaction of a relatively conserved portion of L₁ ORF of HPV.A specific fragment of 394-552 bP and 139-15O bp was amplified by PCR from clinical specimens(cervical carcinoma, genital condylomata,ovarian adenocarcinoma etc.)containing DNA from various HPV types. The digestion pattern of restriction endonuclease Rsa Ⅰ analysis of PCR products by PAGE can be used for typing. This method is simple，sensitive，specific and is suitable for detection and typing for genital HPV types in clinical samples.