Amyloglucosdase was coentrapped with glucose oxidase(GOD)in a poly vinyl alcohol membrane.The multi-enzyme membrane was combined with an oxygen electrode to form a bienzyme electrode,which could be incorporated in a flow injection system to determine maltose,Temperature effect and pH effect on the bienzyme electrode system were investigated.The linear respones range for the multi-enzyme electrode was 0.5-35mmol/L of sugar.The entire response cycle was less than 2min.The variation coeffect (CV)was 1.8%.The bienzyme electrode was used semi-continuously for 10 days，at the end of which period its respones to maltoseremained 60% of its initial value. Methods to extend the life time of the bienzyme electrode and to eliminate the interference of coexisting glucose are discussed.